Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- 42-9005-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 7 Monoclonal Antibody (LP5K), eFluor™ 615, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (or basic) keratin expressed either alone or paired with cytokeratin 19 in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression of cytokeratin 7 in the gastric foveolar, intestinal, and stratified squamous epithelia is extremely low or undetectable. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. In addition, most carcinomas express cytokeratin 7. The coordinated expression of this keratin with cytokeratin 20 is commonly used as a diagnostic marker for a variety of carcinomas. Applications Reported: This LP5K antibody has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: This LP5K antibody has been tested immunocytochemistry on fixed and permeabilized MCF7 cells at less than or equal to 10 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LP5K
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry on fixed and permeabilized MCF-7 cells using 10 µg/mL Mouse IgG2b K Isotype Control eFluor® 615 (left) or 10 µg/mL Anti-Human Cytokeratin 7 eFluor® 615 (right). Nuclei are counterstained with DAPI.