42-9005-82

antibody from Invitrogen Antibodies
Targeting: KRT7 CK7, K2C7, K7, SCL
Provider product page for 42-9005-82
Immunocytochemistry
Immunohistochemistry

Antibody data

Product number
42-9005-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
Cytokeratin 7 Monoclonal Antibody (LP5K), eFluor™ 615, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This LP5K monoclonal antibody reacts with human cytokeratin 7 (K7), a 54-kDa type II (or basic) keratin expressed either alone or paired with cytokeratin 19 in simple epithelia, mesothelium, urothelium, and pseudostratified epithelium. Expression of cytokeratin 7 in the gastric foveolar, intestinal, and stratified squamous epithelia is extremely low or undetectable. Cytokeratins form the intracellular cytoskeletal network that maintains the integrity and stability of cells and tissues. In addition, most carcinomas express cytokeratin 7. The coordinated expression of this keratin with cytokeratin 20 is commonly used as a diagnostic marker for a variety of carcinomas. Applications Reported: This LP5K antibody has been reported for use in immunohistochemical and immunocytochemical staining. Applications Tested: This LP5K antibody has been tested immunocytochemistry on fixed and permeabilized MCF7 cells at less than or equal to 10 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
LP5K
Vial size
100 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
KRT7 protein structure - 42-9005-82 shown in red.