M02416-2

antibody from Boster Biological Technology

Targeting: KRT7 CK7, K2C7, K7, SCL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

M02416-2

Provider

Boster Biological Technology

Product name

Anti-Cytokeratin 7 KRT7 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CYTOKERATIN 7/KRT7 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat CYTOKERATIN 7/KRT7 information: Molecular Weight: 51386 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

GEC-11

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Necroptosis-Related Prognostic Model for Pancreatic Carcinoma Reveals Its Invasion and Metastasis Potential through Hybrid EMT and Immune Escape.

Liu H, Li Z, Zhang L, Zhang M, Liu S, Wang J, Yang C, Peng Q, Du C, Jiang N
Biomedicines 2023 Jun 16;11(6)

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Heme oxygenase-1 gene modified human placental mesenchymal stem cells promote placental angiogenesis and spiral artery remodeling by improving the balance of angiogenic factors in vitro.

Wu D, Liu Y, Liu X, Liu W, Shi H, Zhang Y, Zou L, Zhao Y
Placenta 2020 Sep 15;99:70-77

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Immunomodulatory effect of human amniotic epithelial cells on restoration of ovarian function in mice with autoimmune ovarian disease.

Zhang Q, Huang Y, Sun J, Gu T, Shao X, Lai D
Acta biochimica et biophysica Sinica 2019 Aug 5;51(8):845-855

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Human amniotic epithelial cells inhibit growth of epithelial ovarian cancer cells via TGF‑β1-mediated cell cycle arrest.

Bu S, Zhang Q, Wang Q, Lai D
International journal of oncology 2017 Nov;51(5):1405-1414

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of Cytokeratin 7 expression in HeLa cell lysate (M02416-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 monoclonal antibody (Catalog # M02416-2) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7

Additional image