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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-33104 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33104, RRID:AB_10004030
- Product name
- Rabbit Polyclonal Cytokeratin 7 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cytokeratin 7 Antibody [NBP1-33104] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:10000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of human placenta (paraformaldehyde-fixed frozen sections), using antibody at 1:200 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-33104] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104] - Immunohistochemical characterization of cytokeratin 7 in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens. Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody overnight at 4C and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.
