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- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- NB600-858 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-858, RRID:AB_10000944
- Product name
- Goat Polyclonal Glutamine Synthetase Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Glutamine Synthetase
- Reactivity
- Bacteria
- Host
- Goat
- Vial size
- 1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Glutamine Synthetase Antibody [NB600-858] - Detection of Glutamine Synthetase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.
