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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C59227 - Provider product page
- Provider
- LSBio
- Product name
- GLUL / Glutamine Synthetase Antibody LS-C59227
- Antibody type
- Polyclonal
- Description
- Delipidated and defibrinated
- Reactivity
- Bacteria
- Host
- Goat
- Storage
- Store at 4°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Glutamine Synthetase Polyclonal Antibody-Western blot. Goat anti-Glutamine Synthetase antibody was used to detect Glutamine Synthetase under reducing ® and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti-goat (1:10K in TBS/MB-070) 1 hr RT. Images were collected using the Bio-Rad VersaDoc System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
