Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-C744698 - Provider product page
- Provider
- LSBio
- Product name
- GLUL / Glutamine Synthetase Antibody (Lyophilized) LS-C744698
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Bacteria
- Host
- Goat
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of Goat anti-Glutamine Synthetase antibody. Lane 1: Glutamine Synthetase Reduced. Lane 2: Glutamine Synthetase Non-Reduced. Load: ~1ug of protein per lane. Primary antibody: Glutamine Synthetase antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti goat. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53.3 kDa, 56 kDa for Glutamine Synthetase. Other band(s): Glutamine Synthetase splice variants and isoforms.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of Goat Anti-Glutamine Synthetase primary antibody. Lane 1: Glutamine Synthetase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Glutamine Synthetase antibody at 1:1,000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Observed/Predicted size: 54 kDa, 54 kDa for Glutamine Synthetase (Brevibacterium). Other band(s): None.