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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00002752-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002752-M02, RRID:AB_539170
- Product name
- Mouse Monoclonal Glutamine Synthetase Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GLUL - glutamate-ammonia ligase (glutamine synthetase)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SIRT5 regulation of ammonia-induced autophagy and mitophagy.
Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer.
Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol.
Polletta L, Vernucci E, Carnevale I, Arcangeli T, Rotili D, Palmerio S, Steegborn C, Nowak T, Schutkowski M, Pellegrini L, Sansone L, Villanova L, Runci A, Pucci B, Morgante E, Fini M, Mai A, Russo MA, Tafani M
Autophagy 2015;11(2):253-70
Autophagy 2015;11(2):253-70
Antitumor activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer.
Gross MI, Demo SD, Dennison JB, Chen L, Chernov-Rogan T, Goyal B, Janes JR, Laidig GJ, Lewis ER, Li J, Mackinnon AL, Parlati F, Rodriguez ML, Shwonek PJ, Sjogren EB, Stanton TF, Wang T, Yang J, Zhao F, Bennett MK
Molecular cancer therapeutics 2014 Apr;13(4):890-901
Molecular cancer therapeutics 2014 Apr;13(4):890-901
Optimisation of the quantification of glutamine synthetase and myelin basic protein in cerebrospinal fluid by a combined acidification and neutralisation protocol.
Herbert MK, Kuiperij HB, Verbeek MM
Journal of immunological methods 2012 Jul 31;381(1-2):1-8
Journal of immunological methods 2012 Jul 31;381(1-2):1-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - Analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.Lane 1: GLUL transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6 Analysis of GLUL expression in Jurkat.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - GLUL monoclonal antibody (M02), clone 3B6. Analysis of GLUL expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Glutamine Synthetase Antibody (3B6) [H00002752-M02] - Detection limit for recombinant GST tagged GLUL is approximately 0.03ng/ml as a capture antibody.
