Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [7]
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Validation data
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- Product number
- NBP1-89768 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89768, RRID:AB_11029064
- Product name
- Rabbit Polyclonal Glutamine Synthetase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Glutamine Synthetase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Profiling of atherosclerotic lesions by gene and tissue microarrays reveals PCSK6 as a novel protease in unstable carotid atherosclerosis.
Glutamine fuels a vicious cycle of autophagy in the tumor stroma and oxidative mitochondrial metabolism in epithelial cancer cells: implications for preventing chemotherapy resistance.
Perisic L, Hedin E, Razuvaev A, Lengquist M, Osterholm C, Folkersen L, Gillgren P, Paulsson-Berne G, Ponten F, Odeberg J, Hedin U
Arteriosclerosis, thrombosis, and vascular biology 2013 Oct;33(10):2432-43
Arteriosclerosis, thrombosis, and vascular biology 2013 Oct;33(10):2432-43
Glutamine fuels a vicious cycle of autophagy in the tumor stroma and oxidative mitochondrial metabolism in epithelial cancer cells: implications for preventing chemotherapy resistance.
Ko YH, Lin Z, Flomenberg N, Pestell RG, Howell A, Sotgia F, Lisanti MP, Martinez-Outschoorn UE
Cancer biology & therapy 2011 Dec 15;12(12):1085-97
Cancer biology & therapy 2011 Dec 15;12(12):1085-97
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutamine Synthetase Antibody [NBP1-89768] - Analysis in control (vector only transfected HEK293T lysate) and GLUL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glutamine Synthetase Antibody [NBP1-89768] - Analysis using Anti-GLUL antibody NBP1-89768 (A) shows similar pattern to independent antibody NBP1-89767 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human hippocampus shows strong cytoplasmic positivity in subsets of glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining in human cerebral cortex and kidney tissues using NBP1-89768 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.