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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [10]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-28940 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Glutamine Synthetase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 293T, U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, mouse eye, rat brain. Predicted reactivity: Mouse (94%), Rat (92%), Xenopus laevis (85%), Dog (97%), Pig (96%), Chicken (88%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Phenotypical changes of satellite glial cells in a murine model of G(M1) -gangliosidosis.
Immunohistochemical Characterization of the Nervous System of Culex pipiens (Diptera, Culicidae).
Analysis of mir-9 Expression Pattern in Rat Retina during Postnatal Development.
Phenotypical peculiarities and species-specific differences of canine and murine satellite glial cells of spinal ganglia.
Glutamine synthetase in avian muscle contributes to a positive myogenic response to ammonia compared with mammalian muscle.
Huang B, Zdora I, de Buhr N, Eikelberg D, Baumgärtner W, Leitzen E
Journal of cellular and molecular medicine 2022 Jan;26(2):527-539
Journal of cellular and molecular medicine 2022 Jan;26(2):527-539
Immunohistochemical Characterization of the Nervous System of Culex pipiens (Diptera, Culicidae).
Gregor KM, Becker SC, Hellhammer F, Baumgärtner W, Puff C
Biology 2022 Jan 1;11(1)
Biology 2022 Jan 1;11(1)
Analysis of mir-9 Expression Pattern in Rat Retina during Postnatal Development.
Pöstyéni E, Kovács-Valasek A, Urbán P, Czuni L, Sétáló G Jr, Fekete C, Gabriel R
International journal of molecular sciences 2021 Mar 4;22(5)
International journal of molecular sciences 2021 Mar 4;22(5)
Phenotypical peculiarities and species-specific differences of canine and murine satellite glial cells of spinal ganglia.
Huang B, Zdora I, de Buhr N, Lehmbecker A, Baumgärtner W, Leitzen E
Journal of cellular and molecular medicine 2021 Jul;25(14):6909-6924
Journal of cellular and molecular medicine 2021 Jul;25(14):6909-6924
Glutamine synthetase in avian muscle contributes to a positive myogenic response to ammonia compared with mammalian muscle.
Stern RA, Mozdziak PE
American journal of physiology. Regulatory, integrative and comparative physiology 2019 Jul 1;317(1):R214-R221
American journal of physiology. Regulatory, integrative and comparative physiology 2019 Jul 1;317(1):R214-R221
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamine synthetase using 20 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glutamine synthetase polyclonal antibody (Product # PA5-28940) at a dilution of 1:20,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamine synthetase using 50 µg rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glutamine synthetase polyclonal antibody (Product # PA5-28940) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutamine synthetase using Various whole cell extracts (30 µg). Samples were loaded onto a 10% SDS-PAGE gel and probed with a Glutamine synthetase polyclonal antibody (Product # PA5-28940) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Glutamine Synthetase was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) at a dilution of 1:20000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Glutamine Synthetase was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940). Whole cell extract (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) diluted at 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940). Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) diluted at 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) diluted at 1:50,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of Glutamine Synthetase was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR531952_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of Glutamine Synthetase was performed by loading 20 µg of HeLa wild type (Lane 1), HeLa CAS9 (Lane 2), HeLa Glutamine Synthetase KO (Lane 3) whole cell extracts. The blot was probed with Anti-Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) using 1:5000 dilution and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036). Loss of signal upon CRISPR mediated knockout (KO) confirms that the antibody is specific to Glutamine Synthetase.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Glutamine Synthetase was performed in DIV9 rat E18 primary cortical neurons fixed in 4% paraformaldehyde at RT for 15 min. Green: Glutamine Synthetase Polyclonal Antibody (Product # PA5 28940) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Frozen) analysis of Glutamine-Synthetase was performed in frozen-sectioned adult mouse cerebellum tissue using Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940) at a dilution of 1:250 (Green). Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Glutamine Synthetase was performed in paraffin-embedded adult mouse retina tissue using Green: CRX Polyclonal Antibody (Product # PA5-32182) at a dilution of 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H441 xenograft , using Glutamine Synthetase (Product # PA5-28940) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Glutamine synthetase was performed in IMR32 whole cell extracts using 5 µg of Glutamine Synthetase Polyclonal Antibody (Product # PA5-28940). Samples were transferred to a membrane and probed with Glutamine Synthetase Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
