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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010926-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010926-M01, RRID:AB_536958
- Product name
- Mouse Monoclonal DBF4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DBF4 - DBF4 homolog (S. cerevisiae)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity.
Hughes S, Jenkins V, Dar MJ, Engelman A, Cherepanov P
The Journal of biological chemistry 2010 Jan 1;285(1):541-54
The Journal of biological chemistry 2010 Jan 1;285(1):541-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of DBF4 expression in transfected 293T cell line by DBF4 monoclonal antibody (M01), clone 6G9.Lane 1: DBF4 transfected lysate(76.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: DBF4 Antibody (6G9) [H00010926-M01] - Detection limit for recombinant GST tagged DBF4 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: DBF4 Antibody (6G9) [H00010926-M01] - Analysis of monoclonal antibody to DBF4 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DBF4 Antibody (6G9) [H00010926-M01] - Immunoperoxidase of monoclonal antibody to DBF4 ( Cat # H00010926-M01 ) on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
