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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB100-88115 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-88115, RRID:AB_1216219
- Product name
- Rabbit Polyclonal DBF4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: DBF4 Antibody [NB100-88115] - Detection of Human DBF4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DBF4 antibody NB100-88115 used for IP at 3 mcg/mg lysate. DBF4 was also immunoprecipitated by rabbit anti-DBF4 antibody NB100-88114, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
