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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- NBP1-05208 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-05208, RRID:AB_1556316
- Product name
- Chicken Polyclonal alpha-Internexin Antibody
- Antibody type
- Polyclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot of extract of rat brain stem crude extract stained with NBP1-05208, showing a single strong clean band at approx. 66kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: alpha-Internexin Antibody [NBP1-05208] - Western blot analysis of different tissue lysates using chicken pAb to alpha-Internexin, CPCA-a-Int, dilution 1:10,000 in green: (1) protein standard (red), (2) rat brain, (3) rat spinal cord, (4) mouse brain, (5) mouse spinal cord, (6) cow spinal cord and (7) pig spinal cord. CPCA-a-Int antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66kDa, with some variability among different species.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry Free-Floating: alpha-Internexin Antibody [NBP1-05208] - Analysis of a rat cerebellum section stained with chicken alpha-Internexin pAb , dilution 1:5,000 (red), and costained with mouse MBP mAb, 1:5,000 (Green). Following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The alpha-Internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.
