PA1-10009

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-10009

Provider

Invitrogen Antibodies

Product name

alpha Internexin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

In Western blot, human proteins run at ~68 kDa, while rat and mouse run lower at ~66 kDa. Minor bands at ~150 kDa are probably covalent dimers, and bands at ~50 kDa represent alpha-internexin breakdown products. Stable at 4°C for at least one year. Mix 1:1 with 100% glycerol and store at -20°C for longer term storage.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Chicken/Avian

Isotype

IgY

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Internexin in tissue lysates using an alpha Internexin polyclonal antibody (Product # PA1-10009) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) rat brain, 3) rat spinal cord, 4) mouse brain, 5) mouse spinal cord, 6) cow spinal cord and 7) pig spinal cord. The antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa, with some variability among different species.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha Internexin in a rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an alpha internexin polyclonal antibody (Product # PA1-10009) at a dilution of 1:5,000 as seen in red, and costained with an MBP polyclonal antibody at a dilution of 1:5,000 as seen in green. The alpha Internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells, parallel fibers and the axons of granule cells, while the MBP antibody stains myelin sheathes around axons.