Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA1-10017 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Internexin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- In Western blot, a prominent band at ~66 kDa is observed, as well as smaller lower bands which are apparently degradation products. A minor band at ~150 kDa is also seen, resulting from dimerization of alpha-internexin.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha internexin in whole tissue lysates using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) mouse spinal cord, 3) rat spinal cord, 4) bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha internexin. The alpha internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha internexin in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:2,000 as seen in green, using a GFAP polyclonal antibody (Production # PA1-10004) at a dilution of 1:5,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.