PA1-10017

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

PA1-10017

Provider

Invitrogen Antibodies

Product name

alpha Internexin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

In Western blot, a prominent band at ~66 kDa is observed, as well as smaller lower bands which are apparently degradation products. A minor band at ~150 kDa is also seen, resulting from dimerization of alpha-internexin.

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha internexin in whole tissue lysates using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:10,000 as seen in green. 1) protein standard (red), 2) mouse spinal cord, 3) rat spinal cord, 4) bovine spinal cord. Major bands in the 64-66 kDa range corresponds to alpha internexin. The alpha internexin protein from different species is known to vary slightly in SDS-PAGE molecular weight.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of alpha internexin in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained using an alpha internexin polyclonal antibody (Product # PA1-10017) at a dilution of 1:2,000 as seen in green, using a GFAP polyclonal antibody (Production # PA1-10004) at a dilution of 1:5,000 as seen in red, and with DAPI staining the nuclear DNA in blue. The alpha-internexin antibody selectively stains axons and dendrites of neuronal cells, in particular Purkinje cells and parallel fibers the axons of granule cells. The GFAP antibody labels network of glial cells, such as astrocytes in the granule cell layer and white matter and Bergmann glia in the molecular layer.