PA1-4737

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-4737

Provider

Invitrogen Antibodies

Product name

alpha Internexin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Purifed from natural sources

Description

In Western blot, a band at ~66 kDa is detected. Minor bands of approximately 50 kDa and 150 kDa are also detected, which most likely correspond to alpha-internexin breakdown products and covalent dimers, respectively.

Reactivity

Human, Mouse, Rat, Bovine

Host

Chicken/Avian

Isotype

IgY

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of alpha Internexin in rat cortex lysate showing specific immunolabeling of a band at ~ 66 kDa corresponding to alpha Internexin polyclonal antibody (Product # PA1-4737).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of alpha Internexin in neurons and glia cells showing specific labeling of neuronal processes with alpha Internexin polyclonal antibody (Product # PA1-4737) with a dilution of 1:500 (red), and specific labeling of microglia with Anti-Coronin 1a (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Internexin in rat cerebellum showing labeling of axons of granule cell and dendrites of Purkinje with alpha Internexin polyclonal antibody (Product # PA1-4737) using a dilution of 1:5,000 (red). Myelin sheaths around the axons are labeled with MBP with a dilution of 1:5,000 (green) and additional nuclear staining with DAPI (blue).