MA1-4709

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-4709

Provider

Invitrogen Antibodies

Product name

alpha Internexin Monoclonal Antibody (1D2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

In Western blot, a band at ~66 kDa is detected. The epitope recognized by MA1-4709 (C-terminal non-helical extension of the protein) is unusually resistant to aldehyde fixation, so formalin fixation of histological sections is recommended.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

1D2

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of alpha Internexin in rat cortex lysate showing specific immunolabeling of a ~66 kDa band corresponding to alpha Internexin monoclonal antibody (Product # MA1-4709).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of alpha Internexin in cultured rat CNS cells showing specific labeling of neuronal processes with alpha Internexin monoclonal antibody (Product # MA1-4709) using a dilution of 1:250 (red) and specific labeling of perikarya and dendrites with Anti-Microtubule associated protein 2 using a dilution of 1:2,500 (green).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Internexin in rat cerebellum selectively labeling the axons of granule cells with alpha Internexin monoclonal antibody (Product # MA1-4709) using a dilution of 1:5,000 (green) and colabeled with Anti-Calretinin (red).