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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX130053 - Provider product page
- Provider
- GeneTex
- Product name
- alpha Internexin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Red: alpha Internexin protein stained by alpha Internexin antibody (GTX130053) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 £gg of alpha Internexin antibody (GTX130053).Western blot analysis was performed using alpha Internexin antibody (GTX130053).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
