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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F54522 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Neurofilament-66 Antibody / INA / Alpha Internexin
- Antibody type
- Monoclonal
- Description
- This highly specific Neurofilament-66 antibody is suitable for use in Immunohistochemistry/Immunofluorescence/Western blot applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 257CT7.1.2
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Neurofilament-66 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human SH-SY5Y cell lysate with Neurofilament-66 antibody. Expected molecular weight: 55-66 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse brain lysate with Neurofilament-66 antibody. Expected molecular weight: 55-66 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of brain tissue with Neurofilament-66 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human brain tissue with Neurofilament-66 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
