MA3-16752

antibody from Invitrogen Antibodies

Targeting: INA NEF5, NF-66

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA3-16752

Provider

Invitrogen Antibodies

Product name

alpha Internexin Monoclonal Antibody (1D2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat, Feline

Host

Mouse

Isotype

IgG

Antibody clone number

1D2

Vial size

500 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Internexin in different tissue lysates. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16752 using a dilution of 1:10,000. Antibody in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] pig spinal cord and [7] cow spinal cord. The alpha-Internexin antibody reveals the alpha-internexin protein with apparent molecular weight of 64 to 66 kDa with slight variability among species.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of alpha Internexin in mixed cultures of rat CNS cells. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16752). This antibody (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of alpha Internexin in rat cerebellum sections. Samples were incubated in alpha Internexin monoclonal antibody (Product # MA3-16752) using a dilution of 1:5,000 (Green). Costained with chicken Calretinin pAb, dilution 1:2,000 (Red). Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hrs, cut to 45 µM, and free-floating sections were stained with the above antibodies. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum.