HPA008057

INA antibody from Atlas Antibodies

NEF5, NF-66

Eligible for validation within the Antibodypedia Validation Initiative

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Validation data

Product number: HPA008057
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA008057, RRID:AB_1234583
Product name: Anti-INA
Provider product page: Atlas Antibodies - HPA008057
Antibody type: Polyclonal
Description: Affinity purified using the PrEST antigen as affinity ligand
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: ALDIEIAAYRKLLEGEETRFSTSGLSISGLNPLPN PSYLLPPRILSATTSKVSSTGLSLKKEEEEEEASK VASKKTSQIGESFEEILEETVISTKKTEKSNIEET TISSQ
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4¡C for short term storage. Long time storage is recommended at -20¡C.

Provider	Type	Product Number
- No reagents -

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells and neuropil.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse dentate gyrus shows selective immunoreactivity in a subset of granular cells and their dendrites.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human hippocampus shows distinct positivity in neuronal processes.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse olfactory bulb shows strong positivity in neuronal processes and some cell bodies in the external plexiform layer.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse dorsal tenia tecta shows selective immunoreactivity in a subset of neurons.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunofluorescence staining of mouse medulla shows distinct staining of nerve bundles.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining oh human hippocampus shows strong positivity in neuropil.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebellum shows positivity in purkinje cells.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex shows positivity in neuropil.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human kidney shows very weak cytoplasmic positivity.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of mouse brain shows moderate positivity in of in the hippocampus.
Sample type: MOUSE

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunofluorescence staining of mouse brain shows positivity in of in the cerebral cortex.
Sample type: MOUSE

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human cerebral cortex and kidney tissues using HPA008057 antibody. Corresponding INA RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.