14800-1-AP

antibody from Proteintech Group

Targeting: GOT2 KAT4, KATIV, KYAT4, mitAAT

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

14800-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#14800-1-AP, RRID:AB_2247898

Product name

GOT2 antibody

Antibody type

Polyclonal

Description

KD/KO validated GOT2 antibody (Cat. #14800-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Retention of stress susceptibility in the mdx mouse model of Duchenne muscular dystrophy after PGC-1α overexpression or ablation of IDO1 or CD38.

Johnson EE, Southern WM, Doud B, Steiger B, Razzoli M, Bartolomucci A, Ervasti JM
Human molecular genetics 2024 Mar 20;33(7):594-611

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Effects of gas signaling molecule SO(2) in cardiac functions of hyperthyroid rats.

Yang Q, Yang T, Liu X, Liu S, Liu W, Nie L, Chu C, Yang J
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2024 Mar 1;28(2):129-143

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An engineered biosensor enables dynamic aspartate measurements in living cells.

Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
eLife 2024 Feb 23;12

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Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Li F, Fu X, Luo X, Lin Q, Liang H, Niu Y, Liu L, Li N
Frontiers in microbiology 2023;14:1165491

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Comprehensive analysis of the lysine succinylome in fish oil-treated prostate cancer cells.

Jiang Y, He C, Ye H, Xu Q, Chen X, Chen Y, Sun J, Rocha S, Wang M
Life science alliance 2023 Nov;6(11)

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ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells.

Hu Q, Dai J, Zhang Z, Yu H, Zhang J, Zhu X, Qin Y, Zhang L, Zhang P
Cancer research 2023 May 15;83(10):1646-1665

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Mitochondrial redox adaptations enable alternative aspartate synthesis in SDH-deficient cells.

Hart ML, Quon E, Vigil ABG, Engstrom IA, Newsom OJ, Davidsen K, Hoellerbauer P, Carlisle SM, Sullivan LB
eLife 2023 Mar 8;12

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A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

Southwell N, Primiano G, Nadkarni V, Attarwala N, Beattie E, Miller D, Alam S, Liparulo I, Shurubor YI, Valentino ML, Carelli V, Servidei S, Gross SS, Manfredi G, Chen Q, D'Aurelio M
EMBO molecular medicine 2023 Jul 10;15(7):e16951

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NFYA promotes the anti-tumor effects of gluconeogenesis in hepatocellular carcinoma through the regulation of PCK1 expression.

Tsujimoto G, Ito R, Yoshikawa K, Ueki C, Okada N
Frontiers in cell and developmental biology 2022;10:983599

---

Gaseous signal molecule SO(2) regulates autophagy through PI3K/AKT pathway inhibits cardiomyocyte apoptosis and improves myocardial fibrosis in rats with type II diabetes.

Zhao J, Wu Q, Yang T, Nie L, Liu S, Zhou J, Chen J, Jiang Z, Xiao T, Yang J, Chu C
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2022 Nov 1;26(6):541-556

---

Neddylation inhibition induces glutamine uptake and metabolism by targeting CRL3(SPOP) E3 ligase in cancer cells.

Zhou Q, Lin W, Wang C, Sun F, Ju S, Chen Q, Wang Y, Chen Y, Li H, Wang L, Hu Z, Jin H, Wang X, Sun Y
Nature communications 2022 May 31;13(1):3034

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A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H(2)S oxidation.

Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R
The Journal of biological chemistry 2022 Jan;298(1):101435

---

Identification and Validation of a Nine-Gene Amino Acid Metabolism-Related Risk Signature in HCC.

Zhao Y, Zhang J, Wang S, Jiang Q, Xu K
Frontiers in cell and developmental biology 2021;9:731790

---

Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma.

Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, Niu Y, Xu X, Guo S, Yang Y, Wang Z, Xue X, Gu C, Fang W, Sun J, Yu Y, Wang J
Theranostics 2021;11(12):5650-5674

---

Hepatic mTORC1 signaling activates ATF4 as part of its metabolic response to feeding and insulin.

Byles V, Cormerais Y, Kalafut K, Barrera V, Hughes Hallett JE, Sui SH, Asara JM, Adams CM, Hoxhaj G, Ben-Sahra I, Manning BD
Molecular metabolism 2021 Nov;53:101309

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USP29 coordinates MYC and HIF1α stabilization to promote tumor metabolism and progression.

Tu R, Kang W, Yang M, Wang L, Bao Q, Chen Z, Dong Y, Wang J, Jiang J, Liu H, Qing G
Oncogene 2021 Nov;40(46):6417-6429

---

Aspartate availability limits hematopoietic stem cell function during hematopoietic regeneration.

Qi L, Martin-Sandoval MS, Merchant S, Gu W, Eckhardt M, Mathews TP, Zhao Z, Agathocleous M, Morrison SJ
Cell stem cell 2021 Nov 4;28(11):1982-1999.e8

---

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Wang YP, Sharda A, Xu SN, van Gastel N, Man CH, Choi U, Leong WZ, Li X, Scadden DT
Cell metabolism 2021 May 4;33(5):1027-1041.e8

---

The mitochondrial NADH pool is involved in hydrogen sulfide signaling and stimulation of aerobic glycolysis.

Vitvitsky V, Kumar R, Libiad M, Maebius A, Landry AP, Banerjee R
The Journal of biological chemistry 2021 Jan-Jun;296:100736

---

Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.

Liu M, Wang Y, Yang C, Ruan Y, Bai C, Chu Q, Cui Y, Chen C, Ying G, Li B
The Journal of experimental medicine 2020 Mar 2;217(3)

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Ischemic Neuroprotectant PKCε Restores Mitochondrial Glutamate Oxaloacetate Transaminase in the Neuronal NADH Shuttle after Ischemic Injury.

Xu J, Khoury N, Jackson CW, Escobar I, Stegelmann SD, Dave KR, Perez-Pinzon MA
Translational stroke research 2020 Jun;11(3):418-432

---

Glutamine Metabolism Is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Hamanaka RB, O'Leary EM, Witt LJ, Tian Y, Gökalp GA, Meliton AY, Dulin NO, Mutlu GM
American journal of respiratory cell and molecular biology 2019 Nov;61(5):597-606

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Preventing BRCA1/ZBRK1 repressor complex binding to the GOT2 promoter results in accelerated aspartate biosynthesis and promotion of cell proliferation.

Hong R, Zhang W, Xia X, Zhang K, Wang Y, Wu M, Fan J, Li J, Xia W, Xu F, Chen J, Wang S, Zhan Q
Molecular oncology 2019 Apr;13(4):959-977

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Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.

Chen Q, Kirk K, Shurubor YI, Zhao D, Arreguin AJ, Shahi I, Valsecchi F, Primiano G, Calder EL, Carelli V, Denton TT, Beal MF, Gross SS, Manfredi G, D'Aurelio M
Cell metabolism 2018 May 1;27(5):1007-1025.e5

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Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.

Wang YQ, Wang HL, Xu J, Tan J, Fu LN, Wang JL, Zou TH, Sun DF, Gao QY, Chen YX, Fang JY
Nature communications 2018 Feb 7;9(1):545

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Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.

Cai WF, Zhang C, Wu YQ, Zhuang G, Ye Z, Zhang CS, Lin SC
Cell research 2018 Aug;28(8):865-867

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Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.

Feist M, Schwarzfischer P, Heinrich P, Sun X, Kemper J, von Bonin F, Perez-Rubio P, Taruttis F, Rehberg T, Dettmer K, Gronwald W, Reinders J, Engelmann JC, Dudek J, Klapper W, Trümper L, Spang R, Oefner PJ, Kube D
Nature communications 2018 Apr 17;9(1):1514

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HIF-2α regulates non-canonical glutamine metabolism via activation of PI3K/mTORC2 pathway in human pancreatic ductal adenocarcinoma.

Li W, Chen C, Zhao X, Ye H, Zhao Y, Fu Z, Pan W, Zheng S, Wei L, Nong T, Li Z, Chen R
Journal of cellular and molecular medicine 2017 Nov;21(11):2896-2908

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Glutamine metabolism regulates autophagy-dependent mTORC1 reactivation during amino acid starvation.

Tan HWS, Sim AYL, Long YC
Nature communications 2017 Aug 24;8(1):338

---

Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors.

Kitazawa S, Ebara S, Ando A, Baba Y, Satomi Y, Soga T, Hara T
Oncotarget 2017 Apr 25;8(17):28922-28938

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Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.

Qin S, Zhou Y, Gray L, Kusebauch U, McEvoy L, Antoine DJ, Hampson L, Park KB, Campbell D, Caballero J, Glusman G, Yan X, Kim TK, Yuan Y, Wang K, Rowen L, Moritz RL, Omenn GS, Pirmohamed M, Hood L
Journal of proteome research 2016 Oct 7;15(10):3724-3740

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cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions.

Sun L, Song L, Wan Q, Wu G, Li X, Wang Y, Wang J, Liu Z, Zhong X, He X, Shen S, Pan X, Li A, Wang Y, Gao P, Tang H, Zhang H
Cell research 2015 Apr;25(4):429-44

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 14800-1-AP(GOT2 antibody) at dilution of 1:1200

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells, using GOT2 antibody 14800-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin cancer using 14800-1-AP(GOT2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human skin cancer using 14800-1-AP(GOT2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue

Flow cytometry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The GOT2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human GOT2. This antibody recognizes human,mouse,rat antigen. The GOT2 antibody has been validated for the following applications: ELISA, WB, IHC, IF, FC analysis.

Appendix

14800-1-AP.pdf

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