Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
Reference
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- Product number
- GTX101929 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101929, RRID:AB_1240908
- Product name
- GOT2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice.
Arif A, Terenzi F, Potdar AA, Jia J, Sacks J, China A, Halawani D, Vasu K, Li X, Brown JM, Chen J, Kozma SC, Thomas G, Fox PL
Nature 2017 Feb 16;542(7641):357-361
Nature 2017 Feb 16;542(7641):357-361
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HepG2 10% SDS PAGE GTX101929 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOT2 antibody detects GOT2 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract 10% SDS-PAGEGOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOT2 antibody detects GOT2 protein by western blot analysis.A. 50 ?g rat lung lysate/extract10% SDS-PAGEGOT2 antibody (GTX101929) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 antibody (GTX101929) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 antibody (GTX101929) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 antibody (GTX101929) diluted at 1:500.