MA5-15595

antibody from Invitrogen Antibodies

Targeting: GOT2 KAT4, KATIV, KYAT4, mitAAT

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15595

Provider

Invitrogen Antibodies

Product name

GOT2 Monoclonal Antibody (3E9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15595 targets GOT2 in indirect ELISA, IF and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples. The MA5-15595 immunogen is purified recombinant fragment of human GOT2 expressed in E. Coli. MA5-15595 detects GOT2 which has a predicted molecular weight of approximately 47kDa.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

3E9

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and H(2)S metabolism.

Mellis AT, Misko AL, Arjune S, Liang Y, Erdélyi K, Ditrói T, Kaczmarek AT, Nagy P, Schwarz G
Redox biology 2021 Jan;38:101800

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APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.

Liu C, Zhang X, Huang F, Yang B, Li J, Liu B, Luo H, Zhang P, Zhang H
The Journal of biological chemistry 2012 Aug 24;287(35):29373-83

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using GOT2 monoclonal antibody (Product # MA5-15595) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GOT2 was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GOT2 Monoclonal Antibody (Product # MA5-15595) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Contribution of GOT1 and GOT2 to cellular sulfite production . A. Western Blot of HEK293 lysates confirming knockout of SO, GOT1 and GOT2 (n = 3). B. Sulfite standard curve used for the determination of sulfite concentration from cellular extracts (n = 3). C. Sulfite measurement from cell extracts of WT and SUOX -/- cells with and without addition of purified SO (n = 4). Sulfite levels were normalized to cellular protein concentration. Error bars indicate standard deviation. Two-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: ***