MA5-15595

antibody from Invitrogen Antibodies

Targeting: GOT2 KAT4, KATIV, KYAT4, mitAAT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15595

Provider

Invitrogen Antibodies

Product name

GOT2 Monoclonal Antibody (3E9)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15595 targets GOT2 in IF and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.

Antibody clone number

3E9

Concentration

Conc. Not Determined

References

Submitted references

The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and H(2)S metabolism.

Mellis AT, Misko AL, Arjune S, Liang Y, Erdélyi K, Ditrói T, Kaczmarek AT, Nagy P, Schwarz G
Redox biology 2021 Jan;38:101800

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APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.

Liu C, Zhang X, Huang F, Yang B, Li J, Liu B, Luo H, Zhang P, Zhang H
The Journal of biological chemistry 2012 Aug 24;287(35):29373-83

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GOT2 using GOT2 monoclonal antibody (Product # MA5-15595) in HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Panc-1 (Lane 1), Hep G2 (Lane 2), HCT 116 (Lane 3), Neuro-2a (Lane 4), tissue extracts of Mouse Liver (Lane 5), Mouse Brain (Lane 6) and Mouse Heart (Lane 7). The blot was probed with Anti-GOT2 Monoclonal Antibody (Product # MA5-15595, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 43 kDa band corresponding to GOT2 was observed across cell lines and tissues tested. An additional band at 25 kDa was observed in Mouse Liver and Mouse Heart which can be due to the light chain of IgG.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of GOT2 using GOT2 monoclonal antibody (Product # MA5-15595) in HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH/3T3 (6) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using GOT2 monoclonal antibody (Product # MA5-15595) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of GOT2 was performed using 70% confluent log phase PANC-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GOT2 Monoclonal Antibody (Product # MA5-15595) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytosolic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 Contribution of GOT1 and GOT2 to cellular sulfite production . A. Western Blot of HEK293 lysates confirming knockout of SO, GOT1 and GOT2 (n = 3). B. Sulfite standard curve used for the determination of sulfite concentration from cellular extracts (n = 3). C. Sulfite measurement from cell extracts of WT and SUOX -/- cells with and without addition of purified SO (n = 4). Sulfite levels were normalized to cellular protein concentration. Error bars indicate standard deviation. Two-way ANOVA with Tukey's post-hoc test for pairwise comparisons was performed as indicated. p value: ***