Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-27572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GOT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HepG2, Neuro2A, rat lung.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), tissue extracts of Mouse Liver (Lane 2), Mouse Brain (Lane 3) and Mouse Heart (Lane 4). The blot was probed with Anti-GOT2 Polyclonal Antibody (Product # PA5-27572, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 43 kDa band corresponding to GOT2 was observed across cell line and tissues tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GOT2 Polyclonal Antibody (Product # PA5-27572). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. GOT2 Polyclonal Antibody (Product # PA5-27572) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GOT2 Polyclonal Antibody detects GOT2 protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract.10% SDS-PAGE. GOT2 Polyclonal Antibody (Product # PA5-27572) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GOT2 Polyclonal Antibody detects GOT2 protein by western blot analysis. A. 50 µg rat lung lysate/extract.10% SDS-PAGE. GOT2 Polyclonal Antibody (Product # PA5-27572) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of GOT2 in methanol-fixed HeLa cells using a GOT2 polyclonal antibody (Product # PA5-27572) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- GOT2 Polyclonal Antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. GOT2 Polyclonal Antibody (Product # PA5-27572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GOT2 Polyclonal Antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GOT2 Polyclonal Antibody (Product # PA5-27572) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GOT2 Polyclonal Antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma tissue. GOT2 Polyclonal Antibody (Product # PA5-27572) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2 (Product # PA5-27572) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.