HPA018139

antibody from Atlas Antibodies

Targeting: GOT2 KAT4, KATIV, KYAT4, mitAAT

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA018139

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA018139, RRID:AB_1849903

Product name

Anti-GOT2

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human GOT2, Gene description: glutamic-oxaloacetic transaminase 2, mitochondrial, Alternative Gene Names: KAT4, KATIV, mitAAT, Validated applications: IHC, WB, Uniprot ID: P00505, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

HIF1α-dependent uncoupling of glycolysis suppresses tumor cell proliferation

Urrutia A, Mesa-Ciller C, Guajardo-Grence A, Alkan H, Soro-Arnáiz I, Vandekeere A, Ferreira Campos A, Igelmann S, Fernández-Arroyo L, Rinaldi G, Lorendeau D, De Bock K, Fendt S, Aragonés J
Cell Reports 2024;43(4):114103

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Loss of mitochondrial enzyme GPT2 leads to reprogramming of synaptic glutamate metabolism

Baytas O, Davidson S, Kauer J, Morrow E
Molecular Brain 2024;17(1)

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Requirement of hepatic pyruvate carboxylase during fasting, high fat, and ketogenic diet

Selen E, Rodriguez S, Cavagnini K, Kim H, Na C, Wolfgang M
Journal of Biological Chemistry 2022;298(12):102648

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A Cancer Cell–Intrinsic GOT2–PPARδ Axis Suppresses Antitumor Immunity

Abrego J, Sanford-Crane H, Oon C, Xiao X, Betts C, Sun D, Nagarajan S, Diaz L, Sandborg H, Bhattacharyya S, Xia Z, Coussens L, Tontonoz P, Sherman M
Cancer Discovery 2022;12(10):2414-2433

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Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context

Kerk S, Lin L, Myers A, Sutton D, Andren A, Sajjakulnukit P, Zhang L, Zhang Y, Jiménez J, Nelson B, Chen B, Robinson A, Thurston G, Kemp S, Steele N, Hoffman M, Wen H, Long D, Ackenhusen S, Ramos J, Gao X, Nwosu Z, Galban S, Halbrook C, Lombard D, Piwnica-Worms D, Ying H, Pasca di Magliano M, Crawford H, Shah Y, Lyssiotis C
eLife 2022;11

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Interplay Between Thiamine and p53/p21 Axes Affects Antiproliferative Action of Cisplatin in Lung Adenocarcinoma Cells by Changing Metabolism of 2-Oxoglutarate/Glutamate

Aleshin V, Zhou X, Krishnan S, Karlsson A, Bunik V
Frontiers in Genetics 2021;12

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Tissue of origin dictates GOT1 dependence and confers synthetic lethality to radiotherapy.

Nelson BS, Lin L, Kremer DM, Sousa CM, Cotta-Ramusino C, Myers A, Ramos J, Gao T, Kovalenko I, Wilder-Romans K, Dresser J, Davis M, Lee HJ, Nwosu ZC, Campit S, Mashadova O, Nicolay BN, Tolstyka ZP, Halbrook CJ, Chandrasekaran S, Asara JM, Crawford HC, Cantley LC, Kimmelman AC, Wahl DR, Lyssiotis CA
Cancer & metabolism 2020;8:1

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human liver and bone marrow tissues using HPA018139 antibody. Corresponding GOT2 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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