BAF1141

antibody from R&D Systems

Targeting: SPINT1 HAI, MANSC2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BAF1141

Provider

R&D Systems

Product name

Mouse HAI-1 Biotinylated Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse HAI-1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Role of the vasohibin family in the regulation of fetoplacental vascularization and syncytiotrophoblast formation.

Suenaga K, Kitahara S, Suzuki Y, Kobayashi M, Horie S, Sugawara J, Yaegashi N, Sato Y
PloS one 2014;9(9):e104728

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

HAI-1 in Mouse Intestine. HAI-1 was detected in perfusion fixed frozen sections of mouse intestine using Mouse HAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1141) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.