Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- BAF1141 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse HAI-1 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse HAI-1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with rhSiglec-2 and rhSiglec-3 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Role of the vasohibin family in the regulation of fetoplacental vascularization and syncytiotrophoblast formation.
Suenaga K, Kitahara S, Suzuki Y, Kobayashi M, Horie S, Sugawara J, Yaegashi N, Sato Y
PloS one 2014;9(9):e104728
PloS one 2014;9(9):e104728
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- HAI-1 in Mouse Intestine. HAI-1 was detected in perfusion fixed frozen sections of mouse intestine using Mouse HAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1141) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.