AF1141

antibody from R&D Systems

Targeting: SPINT1 HAI, MANSC2

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF1141

Provider

R&D Systems

Product name

Mouse HAI-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI-1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

NP_058603


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation.

Mikelis CM, Palmby TR, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama YS, Bugge TH, Gutkind JS
The Journal of biological chemistry 2013 Apr 26;288(17):12232-43

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Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.

Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
Cancer research 2013 Apr 15;73(8):2659-70

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Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.

Szabo R, Uzzun Sales K, Kosa P, Shylo NA, Godiksen S, Hansen KK, Friis S, Gutkind JS, Vogel LK, Hummler E, Camerer E, Bugge TH
PLoS genetics 2012;8(8):e1002937

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Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice.

Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B
American journal of respiratory cell and molecular biology 2010 Mar;42(3):286-93

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Potent inhibition and global co-localization implicate the transmembrane Kunitz-type serine protease inhibitor hepatocyte growth factor activator inhibitor-2 in the regulation of epithelial matriptase activity.

Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH
The Journal of biological chemistry 2008 Oct 24;283(43):29495-504

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Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary.

Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK
The Biochemical journal 2008 Jul 15;413(2):251-9

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Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development.

Szabo R, Molinolo A, List K, Bugge TH
Oncogene 2007 Mar 8;26(11):1546-56