Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 14-9960-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-HAI-1 Monoclonal Antibody (9B10), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 9B10 recognizes HAI-1 also known as hepatocyte growth factor activation inhibitor. HAI-1 is a single transmembrane protein of approximately 53 kDa with an extracellular Kunitz-type serine protease inhibitor domain. HAI-1 and HAI-2 are produced in membrane-associated forms, which are secreted as active, proteolytically truncated proteins. HAI can bind to and inhibit HGFA and matripase, which are responsible for converting HGF to an active form. Human HAI-1 transcript is expressed in various human tissues, such as adult placenta, kidney, pancreas, prostate, small intestine, fetal kidney and fetal lung. HAI-1 is a marker for prostate and breast cancers. Applications Reported: This 9B10 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB) under nonreducing conditions only. Applications Tested: This 9B10 antibody has been tested by flow cytometric analysis of human cell line MCF7. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9B10
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Cytotrophoblast, Not Syncytiotrophoblast, Dominates Glycolysis and Oxidative Phosphorylation in Human Term Placenta.
Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
The hepatocyte growth factor regulatory factors in human breast cancer.
Kolahi KS, Valent AM, Thornburg KL
Scientific reports 2017 Feb 23;7:42941
Scientific reports 2017 Feb 23;7:42941
Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
Kolahi K, Louey S, Varlamov O, Thornburg K
PloS one 2016;11(4):e0153522
PloS one 2016;11(4):e0153522
The hepatocyte growth factor regulatory factors in human breast cancer.
Parr C, Watkins G, Mansel RE, Jiang WG
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jan 1;10(1 Pt 1):202-11
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Jan 1;10(1 Pt 1):202-11
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of MCF7 cell line with 0.25 µg of Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human HAI-1 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.