Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34248 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TACR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (95%).
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), A-431 (Lane 2), Caco-2 (Lane 3), MCF7 (Lane 4), NTERA-2 cl.D1 (Lane 5), SK-N-AS (Lane 6) and Hep G2 (Lane 7). The blot was probed with Anti- TACR3 Polyclonal Antibody (Product # PA5-34248, 1:500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). A 50 kDa band corresponding to TACR3 was observed across the cell lines tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed paraffin-embedded human brain, neurons and glia using a TACR3/NK3R polyclonal antibody (Product # PA5-34248). Heat-induced antigen retrieval was performed prior to staining.