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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF5065 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Calretinin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human, mouse, and rat Calretinin in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 5% cross-reactivity with recombinant human Calbindin D.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PlexinA1 is crucial for the midline crossing of callosal axons during corpus callosum development in BALB/cAJ mice.
The nucleus reuniens: a key node in the neurocircuitry of stress and depression.
Estrogen Treatment Reverses Prematurity-Induced Disruption in Cortical Interneuron Population.
AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury.
Hossain MM, Tsuzuki T, Sakakibara K, Imaizumi F, Ikegaya A, Inagaki M, Takahashi I, Ito T, Takamatsu H, Kumanogoh A, Negishi T, Yukawa K
PloS one 2019;14(8):e0221440
PloS one 2019;14(8):e0221440
The nucleus reuniens: a key node in the neurocircuitry of stress and depression.
Kafetzopoulos V, Kokras N, Sotiropoulos I, Oliveira JF, Leite-Almeida H, Vasalou A, Sardinha VM, Papadopoulou-Daifoti Z, Almeida OFX, Antoniou K, Sousa N, Dalla C
Molecular psychiatry 2018 Mar;23(3):579-586
Molecular psychiatry 2018 Mar;23(3):579-586
Estrogen Treatment Reverses Prematurity-Induced Disruption in Cortical Interneuron Population.
Panda S, Dohare P, Jain S, Parikh N, Singla P, Mehdizadeh R, Klebe DW, Kleinman GM, Cheng B, Ballabh P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Aug 22;38(34):7378-7391
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Aug 22;38(34):7378-7391
AAV2-Mediated Transduction of the Mouse Retina After Optic Nerve Injury.
Nickells RW, Schmitt HM, Maes ME, Schlamp CL
Investigative ophthalmology & visual science 2017 Dec 1;58(14):6091-6104
Investigative ophthalmology & visual science 2017 Dec 1;58(14):6091-6104
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human/Mouse/Rat Calretinin by Western Blot. Western blot shows lysates of human brain, mouse brain and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calretinin at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Calretinin in Human Brain. Calretinin was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using 15 µg/mL Goat Anti-Human/Mouse/ Rat Calretinin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5065) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
