PAB20372

antibody from Abnova Corporation

Targeting: CALB2 CAL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20372

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20372, RRID:AB_10965046

Product name

CALB2 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant CALB2.

Antigen sequence

LKGFLSDLLKKANRPYDEPKLQEYTQTILRMFDLN
GDGKLGLSEMSRLLPVQENFLLKFQGMKLTSEEFN
AIFTFYDKDRSGYIDEHELDALLKDLYEKNKKEMN
IQQLTNYRKSVMSLAEAGKLYRKDLEIV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with CALB2 polyclonal antibody (Cat # PAB20372) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with CALB2 polyclonal antibody (Cat # PAB20372) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum with CALB2 polyclonal antibody (Cat # PAB20372) shows strong cytoplasmic and nuclear positivity in Purkinje cells and strong nuclear positivity in cells in molecular layer at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)