- MA5-14540 - Invitrogen Antibodies CALB2 antibody

Submitted references Whole-Brain Neural Connectivity to Lateral Pontine Tegmentum GABAergic Neurons in Mice.

Chen ZK, Yuan XS, Dong H, Wu YF, Chen GH, He M, Qu WM, Huang ZL
Frontiers in neuroscience 2019;13:375

Lats1 and Lats2 are required for the maintenance of multipotency in the Müllerian duct mesenchyme.

St-Jean G, Tsoi M, Abedini A, Levasseur A, Rico C, Morin M, Djordjevic B, Miinalainen I, Kaarteenaho R, Paquet M, Gévry N, Boyer A, Vanderhyden B, Boerboom D
Development (Cambridge, England) 2019 Oct 18;146(20)

Tenascin XB Is a Novel Diagnostic Marker for Malignant Mesothelioma.

Nakayama K, Seike M, Noro R, Takeuchi S, Matsuda K, Kunugi S, Kubota K, Gemma A
Anticancer research 2019 Feb;39(2):627-633

Six1 is essential for differentiation and patterning of the mammalian auditory sensory epithelium.

Zhang T, Xu J, Maire P, Xu PX
PLoS genetics 2017 Sep;13(9):e1006967

Recurrent renal cell carcinoma manifesting as a large intrathoracic fibrotic mass: A case report.

Kim JH, Jeong JH, Park SH, Jeong JS, Ryu YJ, Song SY
Oncology letters 2016 Jun;11(6):3835-3838

Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.

Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95

Leiomyomatosis peritonealis disseminata associated with endometriosis and multiple uterus-like mass: report of two cases.

Carvalho FM, Carvalho JP, Pereira RM, Ceccato BP Jr, Lacordia R, Baracat EC
Clinical medicine insights. Case reports 2012;5:63-8

Gonadoblastoma: Case report of two young patients with isochromosome 12p found in the dysgerminoma overgrowth component in one case.

Changchien YC, Haltrich I, Micsik T, Kiss E, Fónyad L, Papp G, Sápi Z
Pathology, research and practice 2012 Oct 15;208(10):628-32

Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39

Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.

Ustun H, Astarci HM, Sungu N, Ozdemir A, Ekinci C
Diagnostic cytopathology 2011 Jun;39(6):402-8

Perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament.

Ryś J, Karolewski K, Pudełek J, Kruczak A, Wasilewska A, Vogelgesang M, Kojs Z
Polish journal of pathology : official journal of the Polish Society of Pathologists 2008;59(4):211-5

Confusing cases: clear cell but not renal cell lesions in kidney.

Baydar D, Aydin O
Pathology international 2008 Nov;58(11):713-7

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Calretinin was performed by loading 25 µg of U87-MG (lane 1), SK-N-MC (lane 2) and mouse brain (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Calretinin monoclonal antibody (Product # MA5-14540) at a dilution of 1:50 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~29 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Calretinin was achieved by transfecting HT-29 with Calretinin specific siRNAs (Silencer® select Product # s224821, s2325). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the Calretinin knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Calretinin Monoclonal Antibody (SP13) (Product # MA5-14540, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Calretinin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Calretinin was performed using whole cell extracts (30 µg lysate) of HEL 92.1.7 (Lane 1), A-431 (Lane 2), U-2 OS (Lane 3), U-87 MG (Lane 4), HeLa (Lane 5) and tissue extract (30 µg lysate) of Mouse Brain (Lane 6). The blots were probed with Anti-Calretinin Rabbit Monoclonal Antibody (Product # MA5-14540, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 31 kDa band corresponding to Calretinin was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human mesothelioma stained with Calretinin using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and nuclear staining of tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 Lack of outer hair cell formation in Six1 CKO ( Eya1 CreER ) cochlea. (A,B) Immunostaining for Myo7a of cochlea from E18.5 embryos given tamoxifen at E11.5-12.5. (A',B') H&E staining of section from middle region of cochlea indicated by dashed line in A or B respectively. (A"",B"") Higher magnification of boxed region in A or B respectively. (C-F) Co-immunostaining on sections for Myo7a (green) and Calretinin (red). Abb.: dc, Deiters' cells; GER, greater epithelial ridge; ibc, inner border cell; ihc, inner hair cells; iph, inner phalangeal cell; oc, organ of Corti; ohc, outer hair cells; pc, pillar cells; sc, supporting cells. Scale bars: 200 mum in A and B; 50 mum in A',B' and C-F; 30 mum in A"" and B"".

MA5-14540

Antibody data