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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-16328 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calretinin Monoclonal Antibody (SP13)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody is predicted to react with bovine, chicken and rat based on sequence homology. Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is mesothelioma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP13
- Vial size
- 500 µL
- Concentration
- Concentration not determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Ganglion cardiacum or juxtaductal body of human fetuses.
Recurrent renal cell carcinoma manifesting as a large intrathoracic fibrotic mass: A case report.
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Leiomyomatosis peritonealis disseminata associated with endometriosis and multiple uterus-like mass: report of two cases.
Gonadoblastoma: Case report of two young patients with isochromosome 12p found in the dysgerminoma overgrowth component in one case.
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.
Perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament.
Confusing cases: clear cell but not renal cell lesions in kidney.
Kim JH, Cho KH, Jin ZW, Murakami G, Abe H, Chai OH
Anatomy & cell biology 2018 Dec;51(4):266-273
Anatomy & cell biology 2018 Dec;51(4):266-273
Recurrent renal cell carcinoma manifesting as a large intrathoracic fibrotic mass: A case report.
Kim JH, Jeong JH, Park SH, Jeong JS, Ryu YJ, Song SY
Oncology letters 2016 Jun;11(6):3835-3838
Oncology letters 2016 Jun;11(6):3835-3838
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
Sauter JL, Ambaye AB, Mount SL
Diagnostic cytopathology 2015 Sep;43(9):688-95
Diagnostic cytopathology 2015 Sep;43(9):688-95
Leiomyomatosis peritonealis disseminata associated with endometriosis and multiple uterus-like mass: report of two cases.
Carvalho FM, Carvalho JP, Pereira RM, Ceccato BP Jr, Lacordia R, Baracat EC
Clinical medicine insights. Case reports 2012;5:63-8
Clinical medicine insights. Case reports 2012;5:63-8
Gonadoblastoma: Case report of two young patients with isochromosome 12p found in the dysgerminoma overgrowth component in one case.
Changchien YC, Haltrich I, Micsik T, Kiss E, Fónyad L, Papp G, Sápi Z
Pathology, research and practice 2012 Oct 15;208(10):628-32
Pathology, research and practice 2012 Oct 15;208(10):628-32
Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.
Yin WH, Li J, Chan JK
Diagnostic pathology 2012 Apr 12;7:39
Diagnostic pathology 2012 Apr 12;7:39
Primary malignant deciduoid peritoneal mesothelioma: a report of the cytohistological and immunohistochemical appearances.
Ustun H, Astarci HM, Sungu N, Ozdemir A, Ekinci C
Diagnostic cytopathology 2011 Jun;39(6):402-8
Diagnostic cytopathology 2011 Jun;39(6):402-8
Perivascular epithelioid tumor (PEComa) of the falciform/ broad ligament.
Ryś J, Karolewski K, Pudełek J, Kruczak A, Wasilewska A, Vogelgesang M, Kojs Z
Polish journal of pathology : official journal of the Polish Society of Pathologists 2008;59(4):211-5
Polish journal of pathology : official journal of the Polish Society of Pathologists 2008;59(4):211-5
Confusing cases: clear cell but not renal cell lesions in kidney.
Baydar D, Aydin O
Pathology international 2008 Nov;58(11):713-7
Pathology international 2008 Nov;58(11):713-7
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Calretinin using anti-Calretinin Monoclonal Antibody (Product # MA5-16328) in Mesothelioma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 The ganglion cardiacum (ggl) at gestational age 28 weeks (crown-rump length 235 mm). Sagittal sections stained with antibodies to S100 protein (B), smooth muscle actin (SMA) (C) and calretinin (CAL) (D, E). Panels B-E represent nearly sections, with panel E showing a plane 0.1 mm posterior to panel D. In panel B, the ggl surrounds the left laryngeal recurrent nerve (LRN). The ggl is negative for SMA (C) and contains few neurons positive for calretinin (D, E). AO, aorta, BO, Botallo's duct (ductus arteriosus); BR, left main bronchus; LY, lymph nodes; X, vagus nerve. Scale bars=1 mm (A-C), 0.1 mm (D, E).
