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LearnH00005308-M01
antibody from Abnova Corporation
Targeting: PITX2
ARP1, Brx1, IGDS, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RS
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00005308-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005308-M01, RRID:AB_509266
- Product name
- PITX2 monoclonal antibody (M01), clone 2G6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PITX2.
- Antigen sequence
MNCMKGPLHLEHRAAGTKLSAVSSSSCHHPQPLAM
ASVLAPGQPRSLDSSKHRLEVHTISDTSSPEAAEK
DKSQQGKNEDVGAEDPSKKKRQRRQRTHFTSQQLQ
ELEATFQRNRYPDMSTREEIAVWTNLTEARVRVWF
KNRRAKWRKRERNQQAELCKNGFGPQFNGLMQPYD
DMYPGYSYNNWAAKGLTSASLSTKSFPFFNSMNVN
PLSSQSMFSPPNSISSMSMSSSMVPSAVTGVPGSS
LNSLNNLNNLSSPSLNSAVPTPACPYAPPTPPYVY
RDTCNSSLASLRLKAKQHSSFGYASVQNPASNLSA
CQYAVDRPV- Isotype
- IgG
- Antibody clone number
- 2G6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references PITX2 is involved in stress response in cultured human trabecular meshwork cells through regulation of SLC13A3.
Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells.
Strungaru MH, Footz T, Liu Y, Berry FB, Belleau P, Semina EV, Raymond V, Walter MA
Investigative ophthalmology & visual science 2011 Sep 29;52(10):7625-33
Investigative ophthalmology & visual science 2011 Sep 29;52(10):7625-33
Human PRKC apoptosis WT1 regulator is a novel PITX2-interacting protein that regulates PITX2 transcriptional activity in ocular cells.
Acharya M, Lingenfelter DJ, Huang L, Gage PJ, Walter MA
The Journal of biological chemistry 2009 Dec 11;284(50):34829-38
The Journal of biological chemistry 2009 Dec 11;284(50):34829-38
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PITX2 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PITX2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
