H00005308-D01P

antibody from Abnova Corporation

Targeting: PITX2 ARP1, Brx1, IGDS, IHG2, IRID2, Otlx2, RGS, RIEG, RIEG1, RS

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005308-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005308-D01P, RRID:AB_1578831

Product name

PITX2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PITX2 protein.

Antigen sequence

METNCRKLVSACVQLEKDKSQQGKNEDVGAEDPSK
KKRQRRQRTHFTSQQLQELEATFQRNRYPDMSTRE
EIAVWTNLTEARVRVWFKNRRAKWRKRERNQQAEL
CKNGFGPQFNGLMQPYDDMYPGYSYNNWAAKGLTS
ASLSTKSFPFFNSMNVNPLSSQSMFSPPNSISSMS
MSSSMVPSAVTGVPGSSLNSLNNLNNLSSPSLNSA
VPTPACPYAPPTPPYVYRDTCNSSLASLRLKAKQH
SSFGYASVQNPASNLSACQYAVDRPV


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PITX2 expression in transfected 293T cell line (H00005308-T01) by PITX2 MaxPab polyclonal antibody.Lane 1: PITX2 transfected lysate(30.30 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PITX2 MaxPab rabbit polyclonal antibody. Western Blot analysis of PITX2 expression in human liver.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PITX2 and CTNNB1. HeLa cells were stained with anti-PITX2 rabbit purified polyclonal 1:1200 and anti-CTNNB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)