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LearnAM1854a
antibody from Abcepta
Targeting: RPS6KB2
KLS, P70-BETA, p70S6Kb, S6KB, S6Kbeta, S6Kβ, STK14B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AM1854a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AM1854a, RRID:AB_10816997
- Product name
- RPS6KB2 Antibody (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Western blot analysis of lysates from Jurkat, MCF-7, K562, A549, mouse NIH/3T3 cell line (from left to right), using RPS6KB2 Antibody (Cat. #AM1854a). AM1854a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane."
- Primary Ab dilution
- 1:2000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- RPS6KB2 Antibody (Cat. #AM1854a) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the RPS6KB2 antibody detected the RPS6KB2 protein (arrow).(1:2000)
- Primary Ab dilution
- 1:1000~2000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6KB2 Monoclonal( Ascites) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- RPS6KB2 Monoclonal( Ascites) (Cat. #AM1854a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
