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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-52925 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF182 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VLECCHRVCA KCLYKIIDFG DSPQGVIVCP FCRFETCLPD DEVSSLPDDN NILVNLTCGG KGKKCLPENP TELLLTPKRL ASLVSPSHTS SNCLVITIME VQRESSPSLS STPVVEFYRP ASFDSVTTVS HNWTVWNCTS LL Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 99%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RNF182 in control (vector only transfected HEK293T lysate) and RNF182 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a RNF182 Polyclonal Antibody (Product # PA5-52925).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of RNF182 in human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra. Samples were probed using a RNF182 Polyclonal Antibody (Product # PA5-52925).
