A302-773A

antibody from Invitrogen Antibodies

Targeting: PRPF6 ANT-1, bB152O15.1, C20orf14, hPrp6, Prp6, RP60, SNRNP102, TOM, U5-102K

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-773A

Provider

Invitrogen Antibodies

Product name

PRP6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse PRP6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PRP6 antibody A302-773A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. PRP6 was also immunoprecipitated by rabbit anti-PRP6 antibody A302-774A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 15 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human PRP6 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-PRP6 (Cat. No. A302-773A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.