PA3-16736

antibody from Invitrogen Antibodies

Targeting: SSTR2

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Immunoelectron microscopy (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-16736

Provider

Invitrogen Antibodies

Product name

SSTR2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20°C or -80°C if preferred

References

Submitted references

Octreotide inhibits secretion of IGF-1 from orbital fibroblasts in patients with thyroid-associated ophthalmopathy via inhibition of the NF-κB pathway.

Kim SE, Kim J, Lee JY, Lee SB, Paik JS, Yang SW
PloS one 2021;16(4):e0249988

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SSTR2 in U2OS cells fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. Samples were incubated in SSTR2 polyclonal antibody (Product # PA3-16736) using a dilution of 1:50 overnight at 4 °C followed by anti-rabbit DyLight 488 (Green) with a dilution of 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SSTR2 in mouse pancreas. Samples were incubated in SSTR2 polyclonal antibody (Product # PA3-16736) followed by DAB with hematoxylin counterstain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of SSTR2 in paraffin embedded rat pancreas tissue. Samples were incubated in SSTR2 polyclonal antibody (Product # PA3-16736).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 OFs from patients with TAO expressed higher levels of somatostatin receptor type (SSTR) 2. (A) OFs were plated at a concentration of 2 x 10 5 cells/well in a 6-well plate. After 24 hours, the levels of SSTR2 on the cell surface of OFs from control individuals (#111, #116, #155) and patients with TAO (#135, #137, #162) were examined by flow cytometry. The gray open histogram represents staining with isotype control antibodies. (B) Mean fluorescence intensity of SSTR2 was 2.85-fold higher in patients with TAO compared to control individuals.