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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-456-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL7/MCP-3/MARC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL7/MCP-3/MARC in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Eotaxin and recombinant mouse (rm) JE is observed and less than 5% cross-reactivity with rmMCP-5 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q03366- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia.
CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection.
The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
Kwiatkowski K, Popiolek-Barczyk K, Piotrowska A, Rojewska E, Ciapała K, Makuch W, Mika J
Cytokine 2019 Jul;119:202-213
Cytokine 2019 Jul;119:202-213
CCL7 and IRF-7 Mediate Hallmark Inflammatory and IFN Responses following Rhinovirus 1B Infection.
Girkin J, Hatchwell L, Foster P, Johnston SL, Bartlett N, Collison A, Mattes J
Journal of immunology (Baltimore, Md. : 1950) 2015 May 15;194(10):4924-30
Journal of immunology (Baltimore, Md. : 1950) 2015 May 15;194(10):4924-30
The expression of Fn14 via mechanical stress-activated JNK contributes to apoptosis induction in osteoblasts.
Matsui H, Fukuno N, Kanda Y, Kantoh Y, Chida T, Nagaura Y, Suzuki O, Nishitoh H, Takeda K, Ichijo H, Sawada Y, Sasaki K, Kobayashi T, Tamura S
The Journal of biological chemistry 2014 Mar 7;289(10):6438-6450
The Journal of biological chemistry 2014 Mar 7;289(10):6438-6450
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
Szymczak WA, Deepe GS Jr
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):1964-74
Journal of immunology (Baltimore, Md. : 1950) 2009 Aug 1;183(3):1964-74
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL7/MARC and Neutralization by Mouse CCL7/MARC Antibody. Recombinant Mouse CCL7/ MARC (Catalog # 456-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL7/ MARC (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CCL7/MARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-456-NA). The ND50 is typically 7.5-45 µg/mL.
