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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Blocking/Neutralizing [1]
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- Product number
- MAB281-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL8/MCP-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL8/MCP-2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 0.1% cross-reactivity with recombinant human (rh) CCL15 and rmCCL3.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 35509
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Toll-like receptor-1 variant and its characteristic cellular phenotype is associated with severe malaria in Papua New Guinean children.
Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
Manning L, Cutts J, Stanisic DI, Laman M, Carmagnac A, Allen S, O'Donnell A, Karunajeewa H, Rosanas-Urgell A, Siba P, Davis TM, Michon P, Schofield L, Rockett K, Kwiatkowski D, Mueller I
Genes and immunity 2016 Jan-Feb;17(1):52-9
Genes and immunity 2016 Jan-Feb;17(1):52-9
Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages.
Karta MR, Wickert LE, Curran CS, Gavala ML, Denlinger LC, Gern JE, Bertics PJ
The Journal of allergy and clinical immunology 2014 Apr;133(4):1227-1230.e4
The Journal of allergy and clinical immunology 2014 Apr;133(4):1227-1230.e4
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Effect of cognate human CD4+ T cell and endothelial cell interactions upon chemokine production.
Tay SS, McCormack A, Rose ML
Transplantation 2004 Oct 15;78(7):987-94
Transplantation 2004 Oct 15;78(7):987-94
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CCL8/MCP-2 and Neutralization by Human CCL8/MCP-2 Antibody. Recombinant Human CCL8/MCP-2 (Catalog # 281-CP) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL8/MCP-2 (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL8/MCP-2 Monoclonal Antibody (Catalog # MAB281). The ND50 is typically 1-4 µg/mL.
