500-P35-1MG

antibody from Invitrogen Antibodies

Targeting: CCL8 HC14, MCP-2, SCYA8

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P35-1MG

Provider

Invitrogen Antibodies

Product name

MCP-2 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: QPDSVSIPIT CCFNVINRKI PIQRLESYTR ITNIQCPKEA VIFKTKRGKE VCADPKERWV RDSMKHLDQI FQNLKP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MCP-2 (CCL8). Anti-Human MCP-2 (CCL8)-specific antibody was purified by affinity chromatography employing an immobilized Human MCP-2 (CCL8) matrix. Sandwich ELISA: To detect Human MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MCP-2 (CCL8) (500-P35Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MCP-2 (CCL8). Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Rabbit

Vial size

1 mg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

The Global Phosphorylation Landscape of SARS-CoV-2 Infection.

Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ
Cell 2020 Aug 6;182(3):685-712.e19

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Vitamin D [1,25(OH)2D3] Differentially Regulates Human Innate Cytokine Responses to Bacterial versus Viral Pattern Recognition Receptor Stimuli.

Fitch N, Becker AB, HayGlass KT
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 1;196(7):2965-72

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CCL8 is a potential molecular candidate for the diagnosis of graft-versus-host disease.

Hori T, Naishiro Y, Sohma H, Suzuki N, Hatakeyama N, Yamamoto M, Sonoda T, Mizue Y, Imai K, Tsutsumi H, Kokai Y
Blood 2008 Apr 15;111(8):4403-12

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human MCP-2 (CCL8) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MCP-2 Polyclonal Antibody (Product # 500-P35-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MCP-2 Polyclonal Antibody, Biotin (Product # 500-P35BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MCP-2 (CCL8).