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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunoprecipitation [3]
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Validation data
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- Product number
- LS-C197049 - Provider product page
- Provider
- LSBio
- Product name
- NQO1 Antibody (clone 1E3-A6) LS-C197049
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E3-A6
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NQO1 is approximately 0.3 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.
