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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10835 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NQO1 monoclonal antibody, clone 4D12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NQO1.
- Isotype
- IgG
- Antibody clone number
- 4D12
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) against A-549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and HeLa (5) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) against human NQO1 (aa: 134-274) recombinant protein. (Expected MW is 41.3 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human testis (A) and human ovarian cancer (B) tissues using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using NQO1 monoclonal antibody, clone 4D12 (Cat # MAB10835) (green) and negative control (red).
- Validation comment
- Flow Cytometry
