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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX100235 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100235, RRID:AB_1951017
- Product name
- NQO1 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
Submitted references Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk.
A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.
Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer.
Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.
Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin.
M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice.
Baicalein Decreases Hydrogen Peroxide-Induced Damage to NG108-15 Cells via Upregulation of Nrf2.
Aryl hydrocarbon receptor is a target of 17-Allylamino-17-demethoxygeldanamycin and enhances its anticancer activity in lung adenocarcinoma cells.
Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Cai J, Pires KM, Ferhat M, Chaurasia B, Buffolo MA, Smalling R, Sargsyan A, Atkinson DL, Summers SA, Graham TE, Boudina S
Cell reports 2018 Nov 13;25(7):1708-1717.e5
Cell reports 2018 Nov 13;25(7):1708-1717.e5
A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.
Huang KF, Ma KH, Hung YC, Lo LC, Lin KC, Liu PS, Hu MK, Chueh SH
Journal of cellular physiology 2018 Dec;233(12):9594-9610
Journal of cellular physiology 2018 Dec;233(12):9594-9610
Nrf2 inhibition reverses resistance to GPX4 inhibitor-induced ferroptosis in head and neck cancer.
Shin D, Kim EH, Lee J, Roh JL
Free radical biology & medicine 2018 Dec;129:454-462
Free radical biology & medicine 2018 Dec;129:454-462
Cytoplasmic localization of Nrf2 promotes colorectal cancer with more aggressive tumors via upregulation of PSMD4.
Lin PL, Chang JT, Wu DW, Huang CC, Lee H
Free radical biology & medicine 2016 Jun;95:121-32
Free radical biology & medicine 2016 Jun;95:121-32
Lce1 Family Members Are Nrf2-Target Genes that Are Induced to Compensate for the Loss of Loricrin.
Ishitsuka Y, Huebner AJ, Rice RH, Koch PJ, Speransky VV, Steven AC, Roop DR
The Journal of investigative dermatology 2016 Aug;136(8):1656-1663
The Journal of investigative dermatology 2016 Aug;136(8):1656-1663
M1 Muscarinic Receptor Deficiency Attenuates Azoxymethane-Induced Chronic Liver Injury in Mice.
Rachakonda V, Jadeja RN, Urrunaga NH, Shah N, Ahmad D, Cheng K, Twaddell WS, Raufman JP, Khurana S
Scientific reports 2015 Sep 16;5:14110
Scientific reports 2015 Sep 16;5:14110
Baicalein Decreases Hydrogen Peroxide-Induced Damage to NG108-15 Cells via Upregulation of Nrf2.
Yeh CH, Ma KH, Liu PS, Kuo JK, Chueh SH
Journal of cellular physiology 2015 Aug;230(8):1840-51
Journal of cellular physiology 2015 Aug;230(8):1840-51
Aryl hydrocarbon receptor is a target of 17-Allylamino-17-demethoxygeldanamycin and enhances its anticancer activity in lung adenocarcinoma cells.
Chen PH, Chang JT, Li LA, Tsai HT, Shen MY, Lin P
Molecular pharmacology 2013 Mar;83(3):605-12
Molecular pharmacology 2013 Mar;83(3):605-12
Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.
Liu XM, Peyton KJ, Durante W
American journal of physiology. Heart and circulatory physiology 2013 Jun 15;304(12):H1634-43
American journal of physiology. Heart and circulatory physiology 2013 Jun 15;304(12):H1634-43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.A. 50 ?g mouse heart lysate/extract 12% SDS-PAGENQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NQO1 antibody [C2C3], C-term detects NQO1 protein by western blot analysis.A. 50 ?g rat heart lysate/extract 12% SDS-PAGENQO1 antibody [C2C3], C-term (GTX100235) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Molt-4 (GTX27912) B: Raji 12% SDS PAGE GTX100235 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NQO1(GTX100235) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NQO1 antibody [C2C3], C-term detects NQO1 protein at cytoplasm in human rectal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human rectal carcinoma. NQO1 antibody [C2C3], C-term (GTX100235) diluted at 1:500.
