NB200-209

antibody from Novus Biologicals

Targeting: NQO1 DHQU, DIA4, DTD, NMOR1, QR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-209

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-209, RRID:AB_10002706

Product name

Mouse Monoclonal NQO-1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Does not cross-react with NQO2.

Reactivity

Human, Mouse, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Manganese porphyrin, MnTE-2-PyP, treatment protects the prostate from radiation-induced fibrosis (RIF) by activating the NRF2 signaling pathway and enhancing SOD2 and sirtuin activity.

Shrishrimal S, Chatterjee A, Kosmacek EA, Davis PJ, McDonald JT, Oberley-Deegan RE
Free radical biology & medicine 2020 May 20;152:255-270

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MnTE-2-PyP, a manganese porphyrin, reduces cytotoxicity caused by irradiation in a diabetic environment through the induction of endogenous antioxidant defenses.

Chatterjee A, Kosmacek EA, Shrishrimal S, McDonald JT, Oberley-Deegan RE
Redox biology 2020 Jul;34:101542

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Nrf2 Induction Re-establishes a Proper Neuronal Differentiation Program in Friedreich's Ataxia Neural Stem Cells.

La Rosa P, Russo M, D'Amico J, Petrillo S, Aquilano K, Lettieri-Barbato D, Turchi R, Bertini ES, Piemonte F
Frontiers in cellular neuroscience 2019;13:356

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Modulation of Keap1/Nrf2/ARE Signaling Pathway by Curcuma- and Garlic-Derived Hybrids.

Serafini MM, Catanzaro M, Fagiani F, Simoni E, Caporaso R, Dacrema M, Romanoni I, Govoni S, Racchi M, Daglia M, Rosini M, Lanni C
Frontiers in pharmacology 2019;10:1597

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Targeting NRF2 for the Treatment of Friedreich's Ataxia: A Comparison among Drugs.

Petrillo S, D'Amico J, La Rosa P, Bertini ES, Piemonte F
International journal of molecular sciences 2019 Oct 21;20(20)

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Selenium Deficiency Is Associated with Pro-longevity Mechanisms.

Yim SH, Clish CB, Gladyshev VN
Cell reports 2019 May 28;27(9):2785-2797.e3

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Differential response to lead toxicity in rat primary microglia and astrocytes.

Peng J, Zhou F, Wang Y, Xu Y, Zhang H, Zou F, Meng X
Toxicology and applied pharmacology 2019 Jan 15;363:64-71

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Resveratrol ameliorates sepsis-induced acute kidney injury in a pediatric rat model via Nrf2 signaling pathway.

Wang Y, Feng F, Liu M, Xue J, Huang H
Experimental and therapeutic medicine 2018 Oct;16(4):3233-3240

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The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity.

Bricambert J, Alves-Guerra MC, Esteves P, Prip-Buus C, Bertrand-Michel J, Guillou H, Chang CJ, Vander Wal MN, Canonne-Hergaux F, Mathurin P, Raverdy V, Pattou F, Girard J, Postic C, Dentin R
Nature communications 2018 May 29;9(1):2092

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Nrf2-Inducers Counteract Neurodegeneration in Frataxin-Silenced Motor Neurons: Disclosing New Therapeutic Targets for Friedreich's Ataxia.

Petrillo S, Piermarini E, Pastore A, Vasco G, Schirinzi T, Carrozzo R, Bertini E, Piemonte F
International journal of molecular sciences 2017 Oct 18;18(10)

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Effective protection against acute respiratory distress syndrome/sepsis injury by combined adipose-derived mesenchymal stem cells and preactivated disaggregated platelets.

Chen CH, Chen YL, Sung PH, Sun CK, Chen KH, Chen YL, Huang TH, Lu HI, Lee FY, Sheu JJ, Chung SY, Lee MS, Yip HK
Oncotarget 2017 Oct 10;8(47):82415-82429

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The SOD Mimic, MnTE-2-PyP, Protects from Chronic Fibrosis and Inflammation in Irradiated Normal Pelvic Tissues.

Shrishrimal S, Kosmacek EA, Chatterjee A, Tyson MJ, Oberley-Deegan RE
Antioxidants (Basel, Switzerland) 2017 Nov 6;6(4)

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Capillarisin augments anti-oxidative and anti-inflammatory responses by activating Nrf2/HO-1 signaling.

Kim J, Lim J, Kang BY, Jung K, Choi HJ
Neurochemistry international 2017 May;105:11-20

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Targeting the Nrf2/Amyloid-Beta Liaison in Alzheimer's Disease: A Rational Approach.

Simoni E, Serafini MM, Caporaso R, Marchetti C, Racchi M, Minarini A, Bartolini M, Lanni C, Rosini M
ACS chemical neuroscience 2017 Jul 19;8(7):1618-1627

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Equol Attenuates Atherosclerosis in Apolipoprotein E-Deficient Mice by Inhibiting Endoplasmic Reticulum Stress via Activation of Nrf2 in Endothelial Cells.

Zhang T, Hu Q, Shi L, Qin L, Zhang Q, Mi M
PloS one 2016;11(12):e0167020

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Sinomenine activation of Nrf2 signaling prevents hyperactive inflammation and kidney injury in a mouse model of obstructive nephropathy.

Qin T, Du R, Huang F, Yin S, Yang J, Qin S, Cao W
Free radical biology & medicine 2016 Mar;92:90-99

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Sinomenine attenuates renal fibrosis through Nrf2-mediated inhibition of oxidative stress and TGFβ signaling.

Qin T, Yin S, Yang J, Zhang Q, Liu Y, Huang F, Cao W
Toxicology and applied pharmacology 2016 Aug 1;304:1-8

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Cranberry extract-enriched diets increase NAD(P)H:quinone oxidoreductase and catalase activities in obese but not in nonobese mice.

Boušová I, Bártíková H, Matoušková P, Lněničková K, Zappe L, Valentová K, Szotáková B, Martin J, Skálová L
Nutrition research (New York, N.Y.) 2015 Oct;35(10):901-909

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Nrf2 is essential for the anti-inflammatory effect of carbon monoxide in LPS-induced inflammation.

Qin S, Du R, Yin S, Liu X, Xu G, Cao W
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2015 Jul;64(7):537-48

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GRP78 suppresses lipid peroxidation and promotes cellular antioxidant levels in glial cells following hydrogen peroxide exposure.

Suyama K, Watanabe M, Sakabe K, Otomo A, Okada Y, Terayama H, Imai T, Mochida J
PloS one 2014;9(1):e86951

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Estrogen receptor and PI3K/Akt signaling pathway involvement in S-(-)equol-induced activation of Nrf2/ARE in endothelial cells.

Zhang T, Liang X, Shi L, Wang L, Chen J, Kang C, Zhu J, Mi M
PloS one 2013;8(11):e79075

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Histone deacetylase inhibition activates transcription factor Nrf2 and protects against cerebral ischemic damage.

Wang B, Zhu X, Kim Y, Li J, Huang S, Saleem S, Li RC, Xu Y, Dore S, Cao W
Free radical biology & medicine 2012 Mar 1;52(5):928-36

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Antioxidant components of naturally-occurring oils exhibit marked anti-inflammatory activity in epithelial cells of the human upper respiratory system.

Gao M, Singh A, Macri K, Reynolds C, Singhal V, Biswal S, Spannhake EW
Respiratory research 2011 Jul 13;12:92

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Nrf2 and Keap1 abnormalities in non-small cell lung carcinoma and association with clinicopathologic features.

Solis LM, Behrens C, Dong W, Suraokar M, Ozburn NC, Moran CA, Corvalan AH, Biswal S, Swisher SG, Bekele BN, Minna JD, Stewart DJ, Wistuba II
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jul 15;16(14):3743-53

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Effect of tumor necrosis factor-alpha on estrogen metabolism and endometrial cells: potential physiological and pathological relevance.

Salama SA, Kamel MW, Diaz-Arrastia CR, Xu X, Veenstra TD, Salih S, Botting SK, Kumar R
The Journal of clinical endocrinology and metabolism 2009 Jan;94(1):285-93

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Antiviral activity of Nrf2 in a murine model of respiratory syncytial virus disease.

Cho HY, Imani F, Miller-DeGraff L, Walters D, Melendi GA, Yamamoto M, Polack FP, Kleeberger SR
American journal of respiratory and critical care medicine 2009 Jan 15;179(2):138-50

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Heightened endoplasmic reticulum stress in the lungs of patients with chronic obstructive pulmonary disease: the role of Nrf2-regulated proteasomal activity.

Malhotra D, Thimmulappa R, Vij N, Navas-Acien A, Sussan T, Merali S, Zhang L, Kelsen SG, Myers A, Wise R, Tuder R, Biswal S
American journal of respiratory and critical care medicine 2009 Dec 15;180(12):1196-207

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Nitric oxide activation of Keap1/Nrf2 signaling in human colon carcinoma cells.

Li CQ, Kim MY, Godoy LC, Thiantanawat A, Trudel LJ, Wogan GN
Proceedings of the National Academy of Sciences of the United States of America 2009 Aug 25;106(34):14547-51

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Dietary flaxseed enhances antioxidant defenses and is protective in a mouse model of lung ischemia-reperfusion injury.

Lee JC, Bhora F, Sun J, Cheng G, Arguiri E, Solomides CC, Chatterjee S, Christofidou-Solomidou M
American journal of physiology. Lung cellular and molecular physiology 2008 Feb;294(2):L255-65

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trans-Stilbene oxide induces expression of genes involved in metabolism and transport in mouse liver via CAR and Nrf2 transcription factors.

Slitt AL, Cherrington NJ, Dieter MZ, Aleksunes LM, Scheffer GL, Huang W, Moore DD, Klaassen CD
Molecular pharmacology 2006 May;69(5):1554-63

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5-Methoxy-1,2-dimethyl-3-[(4-nitrophenoxy)methyl]indole-4,7-dione, a mechanism-based inhibitor of NAD(P)H:quinone oxidoreductase 1, exhibits activity against human pancreatic cancer in vitro and in vivo.

Dehn DL, Siegel D, Zafar KS, Reigan P, Swann E, Moody CJ, Ross D
Molecular cancer therapeutics 2006 Jul;5(7):1702-9

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Association of NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population.

Zhang J, Schulz WA, Li Y, Wang R, Zotz R, Wen D, Siegel D, Ross D, Gabbert HE, Sarbia M
Carcinogenesis 2003 May;24(5):905-9

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Association of NAD(P)H: quinone oxidoreductase 1 (NQO1) C609T polymorphism with esophageal squamous cell carcinoma in a German Caucasian and a northern Chinese population.

Zhang J, Schulz WA, Li Y, Wang R, Zotz R, Wen D, Siegel D, Ross D, Gabbert HE, Sarbia M
Carcinogenesis 2003 May;24(5):905-9

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Interaction of human NAD(P)H:quinone oxidoreductase 1 (NQO1) with the tumor suppressor protein p53 in cells and cell-free systems.

Anwar A, Dehn D, Siegel D, Kepa JK, Tang LJ, Pietenpol JA, Ross D
The Journal of biological chemistry 2003 Mar 21;278(12):10368-73

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Rapid polyubiquitination and proteasomal degradation of a mutant form of NAD(P)H:quinone oxidoreductase 1.

Siegel D, Anwar A, Winski SL, Kepa JK, Zolman KL, Ross D
Molecular pharmacology 2001 Feb;59(2):263-8

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Rapid polyubiquitination and proteasomal degradation of a mutant form of NAD(P)H:quinone oxidoreductase 1.

Siegel D, Anwar A, Winski SL, Kepa JK, Zolman KL, Ross D
Molecular pharmacology 2001 Feb;59(2):263-8

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Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues.

Siegel D, Ross D
Free radical biology & medicine 2000 Aug;29(3-4):246-53

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Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues.

Siegel D, Ross D
Free radical biology & medicine 2000 Aug;29(3-4):246-53

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Immortalisation of a human diploid fibroblast cell strain: a DT-diaphorase paradox.

Kuehl BL, Brezden CB, Traver RD, Siegel D, Ross D, Renzing J, Rauth AM
The British journal of cancer. Supplement 1996 Jul;27:S19-22

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: NQO-1 Antibody (A180) [NB200-209] - Simple Western lane view shows a specific band for NQO-1 in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO1 in A549 lysates using NB 200-209. Lane 1: 0.5 ug/ml. Lane 2: 2 ug/ml. Exposure: 1 second.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NQO-1 Antibody (A180) [NB200-209] - Antioxidant enzyme synthesis in response to orange oil treatment. Immunoblot analysis demonstrating expression of NQO1 protein at 6, 12 and 24 hrs following 15 min treatment of BEAS-2B cells with the oil preparation or time-matched soy oil control. Representative blots from one of three separate experiments are shown above. Densitometric evaluations of each target protein blot normalized to its corresponding GAPDH for all three experiments are provided below. Bars represent mean +/- SEM. Image collected and cropped by CiteAb from the following publication (http://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-12-92), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Immunohistochemistry showing Anti-NQ01 on formalin fixed paraffin embedded human kidney tissue

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NQO-1 Antibody (A180) [NB200-209] - Analysis of NQO1 in human breast cancer using DAB with hematoxylin counterstain.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - Detection of NQO-1 in Human A549 Cell Line by Flow Cytometry. Human A549 cell line was stained with Mouse Anti- NQO-1 Monoclonal Antibody (Catalog # NB200-209, filled histogram), or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow (Intracellular): NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U87MG cells with NQO1 (A180) antibody NBP2-24917 (blue) along with a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: NQO-1 Antibody (A180) [NB200-209] - An intracellular stain was performed on U-87 cells with NQO-1 [A180] Antibody NB200-209AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.