Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R35927 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NQO1 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific NQO1 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Immunocytochemistry/ELISA applications with human and rat, pig samples.
- Reactivity
- Human, Rat, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the NQO1 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of rat (A) and pig (B) kidney lysate with NQO1 antibody at 1ug/ml. Predicted molecular weight ~30kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of fixed and permeabilized human HepG2 cells with NQO1 antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human kidney tissue with NQO1 antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.