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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00001728-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00001728-M01, RRID:AB_425399
- Product name
- NQO1 monoclonal antibody (M01), clone 1E3-A6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant NQO1.
- Antigen sequence
MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGW
EVVESDLYAMNFNPIISRKDITGKLKDPANFQYPA
ESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQ
WFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRS
KKAVLSITTGGSGSMYSLQGIHGDMNVILWPIQSG
ILHFCGFQVLEPQLTYSIGHTPADARIQILEGWKK
RLENIWDETPLYFAPSSLFDLNFQAGFLMKKEVQD
EEKNKKFGLSVGHHLGKSIPTDNQIKARK- Isotype
- IgG
- Antibody clone number
- 1E3-A6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NQO1 monoclonal antibody (M01), clone 1E3-A6 Western Blot analysis of NQO1 expression in HepG2 ( Cat # L019V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody (M01), clone 1E3-A6.Lane 1: NQO1 transfected lysate(30.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NQO1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of NQO1 transfected lysate using anti-NQO1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with NQO1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
