M00494

antibody from Boster Biological Technology

Targeting: NQO1 DHQU, DIA4, DTD, NMOR1, QR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M00494

Provider

Boster Biological Technology

Product name

Anti-NQO1/Dt Diaphorase Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for DT DIAPHORASE/NQO1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, ICC, WB. Reactive species: Human, Mouse, Rat DT DIAPHORASE/NQO1 information: Molecular Weight: 30868 MW; Subcellular Localization: Cytoplasm.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

AAOB-14

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Polysaccharides extracted from mulberry fruits (Morus nigra L.): antioxidant effect of ameliorating H(2)O(2)-induced liver injury in HepG2 cells.

Li X, Hua Y, Yang C, Liu S, Tan L, Guo J, Li Y
BMC complementary medicine and therapies 2023 Apr 12;23(1):112

---

Leonurine Preconditioning Attenuates Ischemic Acute Kidney Injury in Rats by Promoting Nrf2 Nuclear Translocation and Suppressing TLR4/NF-κB Pathway.

Han L, Chen A, Liu L, Wang F
Chemical & pharmaceutical bulletin 2022;70(1):66-73

---

Tanshinone I inhibits doxorubicin-induced cardiotoxicity by regulating Nrf2 signaling pathway.

Jiang Q, Chen X, Tian X, Zhang J, Xue S, Jiang Y, Liu T, Wang X, Sun Q, Hong Y, Li C, Guo D, Wang Y, Wang Q
Phytomedicine : international journal of phytotherapy and phytopharmacology 2022 Nov;106:154439

---

Cordycepin alleviated metabolic inflammation in Western diet-fed mice by targeting intestinal barrier integrity and intestinal flora.

Chen J, Wang M, Zhang P, Li H, Qu K, Xu R, Guo N, Zhu H
Pharmacological research 2022 Apr;178:106191

---

Linagliptin protects rat carotid artery from balloon injury and activates the NRF2 antioxidant pathway.

Si J, Meng R, Gao P, Hui F, Li Y, Liu X, Yang B
Experimental animals 2019 Feb 26;68(1):81-90

---

Polysaccharide from Ostrea rivularis attenuates reproductive oxidative stress damage via activating Keap1-Nrf2/ARE pathway.

Li S, Song Z, Liu T, Liang J, Yuan J, Xu Z, Sun Z, Lai X, Xiong Q, Zhang D
Carbohydrate polymers 2018 Apr 15;186:321-331

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of NQO1 expression in SH-SY5Y cell lysate (M00494). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO1 monoclonal antibody (Catalog # M00494) overnight at 4?? then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO1

Additional image