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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-15926 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NQO1 Monoclonal Antibody (4D12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15926 targets NQO1 in FACS, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4D12
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NQO1 using a NQO1 monoclonal antibody (Product # MA5-15926) against a human NQO1 (AA: 134-274) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), U-87 MG (Lane 4), SH-SY5Y (Lane 5) and MOLT4 (Lane 6). The blot was probed with Anti-NQO1 Monoclonal Antibody (Product # MA5-15926, 1:1000 dilution) and detected by chemiluminescence using Goat anti- Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 32 kDa band corresponding to NQO1 was detected across the cell lines tested except SH-SY5Y and MOLT4 which showed little or no expression respectively as reported in the literature.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NQO1 using NQO1 monoclonal antibody (Product # MA5-15926) in A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and HeLa (5) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), U-87 MG (Lane 4), SH-SY5Y (Lane 5) and MOLT4 (Lane 6). The blot was probed with Anti-NQO1 Monoclonal Antibody (Product # MA5-15926, 1:1000 dilution) and detected by chemiluminescence using Goat anti- Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 32 kDa band corresponding to NQO1 was detected across the cell lines tested except SH-SY5Y and MOLT4 which showed little or no expression respectively as reported in the literature.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NQO1 using a NQO1 monoclonal antibody (Product # MA5-15926) against a human NQO1 (AA: 134-274) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NQO1 using NQO1 monoclonal antibody (Product # MA5-15926) in A549 (1), SKNES (2), HepG2 (3), MCF-7 (4) and HeLa (5) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded testis tissues using NQO1 monoclonal antibody (Product # MA5-15926) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using NQO1 monoclonal antibody (Product # MA5-15926) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of NIH/3T3 cells using NQO1 monoclonal antibody (Product # MA5-15926) (green) and negative control (red).
